Medizinonline Medizinonline
  • News
    • News
    • Market & Medicine
  • Patients
    • Disease patterns
    • Diagnostics
    • Therapy
  • Partner Content
    • Dermatology
      • Atopic dermatitis and psoriasis news
      • Dermatology News
    • Diabetes
      • Dia-Month Club – Type 2 Diabetes
      • Diabetes in Motion
      • Diabetes Podcasts
    • Gastroenterology
      • IBD matters
      • Forum Gastroenterology
      • Ozanimod: ulcerative colitis
      • Reflux Update
    • Immunology
      • Primary immunodeficiency – exchange of experience
    • Vaccinate
      • Herpes zoster
    • Infektiologie
    • Neurology
      • EXPERT ULTRASONIC: Introduction to ultrasound-guided injection
      • MS News
      • MS Therapy in Transition
    • Oncology
      • Swiss Oncology in motion
    • Orthopedics
      • Osteoporosis in motion
    • Phytotherapie
    • Practice Management
      • Aargau Cantonal Bank
      • Claraspital
    • Psychiatry
      • Geriatric Psychiatry
    • Rheumatology
  • Departments
    • Allergology and clinical immunology
    • General Internal Medicine
    • Anesthesiology
    • Angiology
    • Surgery
    • Dermatology and venereology
    • Endocrinology and Diabetology
    • Nutrition
    • Gastroenterology and Hepatology
    • Genetics
    • Geriatrics
    • Gynecology
    • Hematology
    • Infectiology
    • Cardiology
    • Nephrology
    • Neurology
    • Emergency and intensive care medicine
    • Nuclear Medicine
    • Oncology
    • Ophthalmology
    • ORL
    • Orthopedics
    • Pediatrics
    • Pharmacology and toxicology
    • Pharmaceutical medicine
    • Phlebology
    • Physical medicine and rehabilitation
    • Pneumology
    • Prevention and health care
    • Psychiatry and psychotherapy
    • Radiology
    • Forensic Medicine
    • Rheumatology
    • Sports Medicine
    • Traumatology and trauma surgery
    • Tropical and travel medicine
    • Urology
    • Dentistry
  • CME & Congresses
    • CME continuing education
    • Congress Reports
    • Congress calendar
  • Practice
    • Noctimed
    • Practice Management
    • Jobs
    • Interviews
  • Log In
  • Register
  • My account
  • Contact
  • English
    • Deutsch
    • Français
    • Italiano
    • Português
    • Español
  • Publications
  • Contact
  • Deutsch
  • English
  • Français
  • Italiano
  • Português
  • Español
Subscribe
Medizinonline Medizinonline
Medizinonline Medizinonline
  • News
    • News
    • Market & Medicine
  • Patienten
    • Krankheitsbilder
    • Diagnostik
    • Therapie
  • Partner Content
    • Dermatology
      • Atopic dermatitis and psoriasis news
      • Dermatology News
    • Diabetes
      • Dia-Month Club – Type 2 Diabetes
      • Diabetes in Motion
      • Diabetes Podcasts
    • Gastroenterology
      • IBD matters
      • Forum Gastroenterology
      • Ozanimod: ulcerative colitis
      • Reflux Update
    • Immunology
      • Primary immunodeficiency – exchange of experience
    • Vaccinate
      • Herpes zoster
    • Infektiologie
    • Neurology
      • EXPERT ULTRASONIC: Introduction to ultrasound-guided injection
      • MS News
      • MS Therapy in Transition
    • Oncology
      • Swiss Oncology in motion
    • Orthopedics
      • Osteoporosis in motion
    • Phytotherapie
    • Practice Management
      • Aargau Cantonal Bank
      • Claraspital
    • Psychiatry
      • Geriatric Psychiatry
    • Rheumatology
  • Departments
    • Fachbereiche 1-13
      • Allergology and clinical immunology
      • General Internal Medicine
      • Anesthesiology
      • Angiology
      • Surgery
      • Dermatology and venereology
      • Endocrinology and Diabetology
      • Nutrition
      • Gastroenterology and Hepatology
      • Genetics
      • Geriatrics
      • Gynecology
      • Hematology
    • Fachbereiche 14-26
      • Infectiology
      • Cardiology
      • Nephrology
      • Neurology
      • Emergency and intensive care medicine
      • Nuclear Medicine
      • Oncology
      • Ophthalmology
      • ORL
      • Orthopedics
      • Pediatrics
      • Pharmacology and toxicology
      • Pharmaceutical medicine
    • Fachbereiche 26-38
      • Phlebology
      • Physical medicine and rehabilitation
      • Phytotherapy
      • Pneumology
      • Prevention and health care
      • Psychiatry and psychotherapy
      • Radiology
      • Forensic Medicine
      • Rheumatology
      • Sports Medicine
      • Traumatology and trauma surgery
      • Tropical and travel medicine
      • Urology
      • Dentistry
  • CME & Congresses
    • CME continuing education
    • Congress Reports
    • Congress calendar
  • Practice
    • Noctimed
    • Practice Management
    • Jobs
    • Interviews
Login

Sie haben noch keinen Account? Registrieren

  • Test procedure

Breast cancer early detection using biomarkers – where do we stand?

    • Congress Reports
    • Genetics
    • Gynecology
    • Oncology
    • RX
    • Studies
  • 3 minute read

Early detection of breast cancer still offers the best chance of cure. Mammography is the benchmark for screening, but is subject to technical, logistical, and diagnostic limitations. For this reason, research is being carried out at full speed on various test methods to detect the disease as early as possible.

An effective and accurate blood test to detect early stages of disease should increase the early detection rate for breast cancer. Therefore, a series of lipid studies were performed in early breast cancer patients and the datasets were combined using machine learning-based analysis to test whether these profiles in plasma could detect breast cancer [1]. Blood samples were collected from women with stage 0-IV breast cancer (4 separate cohorts) and age- and BMI-matched breast cancer-free controls. Lipids from plasma-enriched extracellular vesicles were extracted and analyzed by high-resolution, precision mass LC-MS. Commercially available software was used to annotate and quantify >400 manually curated lipid species. After variable selection, a lipid signature was identified to distinguish breast cancer samples from control samples. Plasma samples from women with breast cancer were discriminated from controls with an average cross-validated accuracy of 0.81 and an average AUC of 0.84 across 4 cohorts. An optimized cross-cohort subset of early-stage IDC, DCIS, and ILC was distinguished from controls with a cross-validated AUC of 0.90, sensitivity of 0.88, and specificity of 0.82 (201 early-stage breast cancer, 199 controls). For this optimized cohort, our assay achieved a sensitivity of 0.71 with a prescribed specificity of 0.90 and a sensitivity of 0.89 with a prescribed specificity of 0.80, respectively. Accordingly, the study demonstrated high sensitivity and specificity of a lipid biomarker signature with potential for early detection of breast cancer.

When the use of bisphosphonates is indicated

Amplification of the transcription factor MAF gene has been associated with increased bone metastases in breast cancer. In addition, patients without MAF amplification in the primary tumor were shown to be more likely to benefit from adjuvant bisphosphonates. A genomic signature could identify patients who lack MAF amplification as candidates for adjuvant bisphosphonates. Therefore, genes that could predict MAF amplification status were investigated [2]. Because MAF amplification is associated with a high risk of bone metastases, a 70-gene signature for risk of distant recurrence (MammaPrint/MP) and an 80-gene signature for molecular subtyping (BluePrint/BP) were used to stratify patient groups. A total of 166 BC patients were enrolled in this pilot cohort. Fluorescence in situ hybridization was performed to detect MAF copy number. A signal-to-core ratio (SNR) of ≥2.5 was used as the threshold for MAF-amplified (MAF+). Differential gene expression analysis was performed with R limma using whole-genome microarray data. MAF+ and MAF- (SNR<2.5) were compared in all patients and in patients matched by MP/BP to balance high-risk groups. Differentially expressed genes (DEGs) were defined as absolute fold change ≥2 and adjusted p-value <0.05. Prediction of MAF amplification based on gene expression was performed using a correlation-based metric with a training set and 1179 stage I-III BC patients from the FLEX study (NCT03053193), which includes MP/BP assays and full transcriptome data.

Of the 166 patients, 12% were MAF+ and 88% were MAF-. Of the MAF+ patients, 95% were MP high-risk patients, as expected based on the association of MAF amplification and bone metastases, as opposed to 29% of MAF- patients. Of note, there was no significant correlation between amplification and MAF gene expression, underscoring the importance of using other genes to predict MAF amplification. When comparing the whole transcriptome of MAF+ and MAF- patients, 48 DEGs were found. From the MP/BP comparisons, genes with a ≥2-fold change were included in the final group of 57 genes, with enrichment of genes encoding C-X-C motif chemokine ligands and S100 calcium binding protein. The 57-gene classifier for MAF status achieved 92% accuracy, 94% specificity, and 75% sensitivity in the training group. Interestingly, when the classifier was applied to the FLEX cohort, 12% MAF+ cases were identified, similar to the training group.

Congress: ASCO Annual Meeting

 

Literature:

  1. Kehelpannala C, Pascovici D, Li D, et al: Detection of early-stage breast cancer in women by plasma lipidomic profiling. J Clin Oncol 40, 2022 (suppl 16; abstr 554).
  2. Nasrazadani A, Gomez Marti JL, Hyder T, et al: Investigation of a genomic signature for transcription factor MAF gene amplification and lack of bisphosphonate benefit in early breast cancer. J Clin Oncol 40, 2022 (suppl 16; abstr 559).

 

InFo ONCOLOGY & HEMATOLOGY 2022; 10(3): 20 (published 6/20/22, ahead of print).

Autoren
  • Leoni Burggraf
Publikation
  • InFo ONKOLOGIE & HÄMATOLOGIE
Related Topics
  • ASCO
  • Biomarker
  • Breast Cancer
Previous Article
  • Mast cell activation syndrome (MCAS)

Practice-relevant findings up-to-date

  • Allergology and clinical immunology
  • Congress Reports
  • Education
  • General Internal Medicine
  • RX
View Post
Next Article
  • Type 2 diabetes - update for the family doctor

Aim for multifactorial rather than glucocentric treatment

  • Cardiology
  • Congress Reports
  • Endocrinology and Diabetology
  • General Internal Medicine
  • Nephrology
  • Prevention and health care
  • RX
  • Studies
View Post
You May Also Like
View Post
  • 10 min
  • Cutaneous lupus erythematosus

From diagnostic workup to successful treatment

    • Congress Reports
    • Dermatology and venereology
    • RX
    • Studies
View Post
  • 4 min
  • Guideline update

Dietary recommendations for Crohn’s disease and colitis

    • Education
    • Gastroenterology and Hepatology
    • General Internal Medicine
    • Nutrition
    • RX
    • Studies
View Post
  • 4 min
  • Circadian rhythm during Ramadan

Disturbed sleep patterns lead to metabolic stress

    • Education
    • Endocrinology and Diabetology
    • General Internal Medicine
    • Nutrition
    • RX
    • Studies
View Post
  • 6 min
  • Case Report

Breast cancer metastases in the bladder

    • Cases
    • Education
    • Gynecology
    • Oncology
    • RX
    • Urology
View Post
  • 11 min
  • Findings from the ALS Symposium 2024 in Montreal

Current and future approaches in the treatment of amyotrophic lateral sclerosis (ALS)

    • Congress Reports
    • Interviews
    • Neurology
    • RX
    • Studies
View Post
  • 8 min
  • Efficacy, mechanism of action and clinical effects

Garlic and high blood pressure

    • Cardiology
    • Education
    • General Internal Medicine
    • Pharmaceutical medicine
    • Phytotherapy
    • RX
    • Studies
View Post
  • 7 min
  • Moderate to severe chronic hand eczema

Delgocitinib cream – first topical pan-JAK inhibitor approved in Switzerland

    • Allergology and clinical immunology
    • Dermatology and venereology
    • Education
    • Infectiology
    • Market & Medicine
    • Pharmacology and toxicology
    • RX
View Post
  • 9 min
  • Wild-type transthyretin amyloid cardiomyopathy (ATTRwt-CA)

Diagnosis at an early stage, prognostic classifications and outlook

    • Cardiology
    • Education
    • RX
    • Studies
Top Partner Content
  • Forum Gastroenterology

    Zum Thema
  • Herpes zoster

    Zum Thema
  • Dermatology News

    Zum Thema
Top CME content
  • 1
    Patience, knowledge and persistence in therapy
  • 2
    Sarcopenia and malnutrition in the context of pneumological rehabilitation
  • 3
    Medical and psychosocial perspectives
  • 4
    New nomenclature for non-alcoholic fatty liver disease
  • 5
    Examinations and considerations before therapy

Newsletter

Sign up and stay up to date

Subscribe
Medizinonline
  • Contact
  • General terms and conditions
  • Imprint

Input your search keywords and press Enter.